Ecological notes of the alien species Godiva quadricolor (Gastropoda: Nudibranchia) occurring in Faro Lake (Italy)

The first record from Sicily of the introduced facelinid nudibranch Godiva quadricolor allowed the detection of trophic relationships with the polycerid Polycera hedgpethi, another non-native nudibranch, and with two bryozoan species, namely the naturalized Cheilostomatida Bugula neritina and the cryptogenic Ctenostomatida Amathia verticillata. The settlement of both nudibranchs was presumably promoted by a trophic shift of P. hedgpethi from the natural prey B. neritina towards the largely available and not exploited A. verticillata. This short food web, without evident links with native fauna and having G. quadricolor as the top predator, is described. A DNA barcoding approach was used to confirm the identity of this facelinid species and to explore the possible genetic divergence occurring among the samples analysed.     

基于DNA-AuNCs的荧光传感新方法灵敏检测胰蛋白酶活性

以DNA模板金纳米簇(DNA-AuNCs)作为荧光探针,发展了一种灵敏检测胰蛋白酶活性的荧光分析新方法.在胰蛋白酶存在条件下,牛血清白蛋白水解成多肽片段,释放出游离的半胱氨酸残基.半胱氨酸残基与金纳米簇通过Au-S键形成非荧光的配合物,导致金纳米簇的荧光强度显著降低.该方法对胰蛋白酶的线性检测范围为0.1～2.0mg/L,检出限为20μg/L(R_(SN)=3).此外,该方法被成功应用于人血清样品中胰蛋白酶含量的测定.     

Comparative genetic differentiation study of three coexisting mangrove crabs in western Atlantic

ABSTRACT

A multispecies approach may reveal the factors influencing the genetic differentiation of coexisting species along a wide geographic area. Our aim was to compare the genetic differentiation of mangrove crabs coexisting along the western Atlantic in order to verify if there are common barriers, using two mitochondrial DNA markers to quantify genetic differentiation, variance and diversity. In addition, we included a mismatch distribution to check demographic history. Our data revealed that species with similar pelagic larval duration had either high genetic differentiation or the absence of genetic structure. These results can be explained by factors that contribute to genetic differentiation, such as the presence of large estuarine areas (acting as barriers), ocean currents, and larval behaviour. Also, historical events that promoted the isolation of some areas in the past, such as glacial cycles during the Pleistocene, could have caused the observed high levels of genetic divergence in some species.     

API8108A语音芯片在人机交互系统中的应用

本文详细介绍了 APL US公司的一次性可编程语音芯片 API810 8A的基本结构及工作原理 ,并讲述了其与单片机的接口技术和在人机交互系统中的应用     

基于GSM的盆栽土壤湿度检测与远程控制

针对盆栽灌溉系统的相关问题,提出一种基于GSM的盆栽土壤湿度检测及远程控制灌溉系统,由C52单片机搭建的湿度检测电路、显示模块和基于PLC的手机远程WiFi控制灌溉模块组成,检测模块将湿度信息显示在显示模块上,并通过GSM通信技术将湿度信息反馈给客户手机,以便客户通过手机控制灌溉模块进行灌溉,最后通过实验论证了系统的可行性与合理性.     

高校计算机实验室出保故障设备解决方法之探索

如何解决好出保故障设备一直是各高校计算机实验室普遍存在的并且难以根治的问题。在新时期下,从高校计算机实验室改革创新的需要和实验队伍的长远发展来看,提高实验技术人员设备维修水平,逐步使其由一般的常规维修向专业化维修过渡是一个科学的、合理的解决方法,并藉此延长计算机及其相关设备的使用寿命和报废期限,弥补高校设备管理之缺陷。     

基于TNY268的反激式LED恒流驱动开关电源设计

LED照明是提高照明质量、节约能源的重要举措。可靠高效的LED恒流驱动电源设计是充分发挥LED照明优势的关键所在。开关电源被誉为高效节能型电源,它非常适合做LED恒流驱动电源,现已成为LED驱动的主要电源。文中设计了一款基于TNY268的反激式小功率开关电源,该电路结构简单,输出电压和恒流驱动值均可随意设定;并依据设计制作了样机,对样机进行了参数测试。实验结果证明:输出电压对输入电压的变化适应力强,输出精度和效率较高。     

新颖场强测试仪的分析与设计

分析了高频无线信号在自由空间的传播规律及接收端场强信号的计算方法,设计了一种新颖高精度、便携带、稳定性好的场强测试仪.该系统以MSP430G2553为核心,通过高放、检波、低放等电路,将检测到的无线信号经过处理后输给单片机,MSP430G2553中AD10将检测到的场强信号转变为数字信号,并在LCD12864上显示场强信号的大小,实测结果表明该系统具有良好的测试精度.     

大草履虫总DNA的提取方法比较

快速而稳定地提取大草履虫的基因组DNA是进行后续分子生物学研究的前提.文章通过比较传统SDS法、CTABⅠ法和CTABⅡ法等提取大草履虫DNA的效果,进一步优化提取方法,获得快速稳定地提取大草履虫基因组DNA的方法.结果表明:用CTAB I法提取的DNA浓度低,杂质较多;CTABII提取的DNA电泳时检测不出条带;而SDS法提取的DNA在电泳时带型较整齐,无降解,且DNA的得率和纯度较高,但仍有蛋白质污染.针对蛋白质的去除,用SDS-蛋白酶K法和SDS-NaAc法来提取DNA,经过试验发现这2种方法都能够很好地去除蛋白质,所获DNA样品的纯度和得率都能用于进一步分子试验,而SDS-NaAc法更经济适用.     

氯化1-辛基-3-甲基咪唑对EMT6细胞的毒性及其DNA损伤作用

研究了氯化1-辛基-3-甲基咪唑([C8mim][Cl])对EMT6细胞的毒性大小及其可能的遗传机制.不同浓度(0.06、0.25、1.00mmol·L-1)的[C8mim][Cl]对EMT6细胞染毒12h,WST-1比色法检测了细胞活力,ELISA方法检测了Bcl-2蛋白的表达,Hoechst 33342染色检测了细胞核形态的变化,单细胞凝胶电泳(SCGE)检测了细胞DNA的损伤,流式细胞仪检测了细胞周期和细胞DNA的含量.结果显示,经[C8mim][Cl]染毒后,EMT6细胞活力下降,并且呈剂量依赖关系.当[C8mim][Cl]浓度高于0.25mmol·L-1时,与对照相比,差异显著.[C8mim][Cl]染毒使Bcl-2蛋白表达下调,引起了细胞核形态改变,造成了DNA的断裂损伤和含量下降,诱导了细胞凋亡.从而可以得出结论,DNA损伤参与了[C8mim][Cl]诱导的细胞凋亡.